Automated Trapping Column Exchanger for High-Throughput Nanoflow Liquid Chromatography

High-Pressure Open-Channel On-Chip Electroosmotic Pump for Nanoflow High Performance Liquid Chromatography

Here, we construct an open-channel on-chip electroosmotic pump capable of generating pressures up to ∼170 bar and flow rates up to ∼500 nL/min, adequate for high performance liquid chromatographic (HPLC) separations. A great feature of this pump is that a number of its basic pump units can be connected in series to enhance its pumping power; the output pressure is directly proportional to the n...

Column Liquid Chromatography

Automated determination of fluvoxamine in plasma by column-switching high-performance liquid chromatography.

A column-switching system with high-performance liquid-chromatographic separation and ultraviolet detection is described for automated determination of fluvoxamine in human plasma or serum. Samples were injected and the drug was retained in a clean-up column [20 x 4.6 mm (i.d.)] filled with C8 reversed-phase material (10-micron particles). After unwanted material was washed out, the drug was el...

Automated pre-column derivatization and its application to amino-acid analysis using high-performance liquid chromatography

Although chemical modification of analytes is a well-accepted adjunct to chromatography, little attention had been paid to the automation of pre-column derivatization techniques. An automated pre-column system would offer advantages when analyses are performed using labile derivatives; and also when the time between injections is long and so staff time spent on achieving maximum analytical outp...

High Performance Liquid Chromatography Coupled with Pre-column Derivatization for Determination of Oxidized Glutathione Level in Rats Exposed to Paraquat

Glutathione (GSH) is one of the most important antioxidants that plays an essential role in detoxification of reactive oxygen species (ROS) which oxidizes to glutathione disulfide (GSSG). Paraquat (PQ), awidely used herbicide, causes pulmonary injury with the productionof ROS. Excessive ROS accumulation as a consequence of PQ exposure are frequently targeted by GSH thereby oxidative stress lead...